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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and <t>MAML1</t> constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.
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Fig. 3. E6 and MAML1 constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.

Journal: Virology

Article Title: Cottontail rabbit papillomavirus E6 proteins: Interaction with MAML1 and modulation of the Notch signaling pathway.

doi: 10.1016/j.virol.2022.08.014

Figure Lengend Snippet: Fig. 3. E6 and MAML1 constructs. In co-immunoprecipitation, E6s are N-terminally tagged with FLAG-HA while hMAML1 obtained from Addgene are N-terminally tagged with V5. All E6s and hMAML1 variants constructs used in FACS-FRET are N-terminally tagged with mTagBFP2 or EYFP, respectively as described. The figure is generated from CorelDraw®X7, version 17.5.0.907.

Article Snippet: MAML1 plasmid, pHAGE-N-V5-hMAML1-FL (Addgene #37048) (Tan et al., 2012) and pHAGE-N-V5-hMAML1 (1–990) (Addgene plasmid #37049) (Tan et al., 2012) were used for co-immunoprecipitation.

Techniques: Construct, Immunoprecipitation, Generated